The Renishaw Diagnostics system has three components as shown in Figure 1. The assay requires extracted nucleic acid from a patient sample for amplification by polymerase chain reaction (PCR) to work in the MDx diagnostics and research assays.
Figure 1: A flow diagram illustrating the main components of the Renishaw Diagnostics assay system.
Specific PCR primers and probes are designed to detect multiple targets from a patient sample. One primer from each set is modified to allow capture of amplified DNA on micro beads. Specific probes are hybridised to the PCR products and captured. The probe is released from the micro bead and analysed by SERRS detection. Each probe has a unique fingerprint and this is read by the detector for rapid detection of multiple pathogenic targets.
