The RenDx Multiplex Assay System provides a new approach to multiplex DNA detection. Starting from the user's existing extraction and amplification methods, the assay utilises purpose-designed instrumentation to automate the processing and detection of up to 9 targets in a single sample.
All post-hybridisation steps are carried out in the RenDx SP-1000; a robotic sample processor, purpose-built for use with all RenDx assays. Samples are then transferred to the RenDx SA-1000 for analysis using the highly sensitive and selective technique of surface enhanced resonance Raman scattering (SERRS).
Nucleic acid extraction & amplification
The RenDx multiplex assay requires nucleic acid(s) to be extracted from the sample under investigation. There is no need for organisms to be cultured and existing extraction methods can be used. Where RNA may be present (if viral organisms are anticipated) then a reverse transcription step is required in order to prepare the template DNA.
Biotinylation, addition of probes & hybridisation
Biotin labels are incorporated into the prepared DNA templates during the PCR stage and the biotinylated PCR product is then denatured. Specially selected and optimised multiplex SERRS probes are then added and, if DNA from a target organism is present, the appropriate probe is hybridised to the target DNA. Samples are then transferred to the RenDx SP-1000. At this point in the assay a mixture of bound and residual, un-bound probes is present in the matrix.
Elution (releasing of probes)
The probes are then eluted and transferred to a clean 96-well detection plate whilst the magnetic beads and attached DNA are discarded.